Plasmid Preparation:Article Title: Robust and flexible platform for directed evolution of yeast genetic switches
Article Snippet: .. Plasmid name Relevant features Source pACYCDuetTM -1 Expression vector for E. coli Novagen pGK415 Expression vector containing pPGK1, TPGK1, the CEN/ARS replication origin, and the LEU2 marker (2) pRS403 Integration vector (without yeast replication origin) with the HIS3 marker ATCC pRS405 Integration vector (without yeast replication origin) with the LEU2 marker ATCC pRS406 Integration vector (without yeast replication origin) with the URA3 marker ATCC pRS416 Expression vector containing the CEN/ARS replication origin and URA3 marker ATCC pATP403 Genome integration vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the HIS3 marker (3) pATP406 Genome integration vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the URA3 marker (3) pATP426 Expression vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the URA3 marker (3) pATP403red Modified version of pATP403 with an extended HIS3 marker This study pRS403red Modified version of pRS403 with an extended HIS3 marker This study pRS405red Modified version of pRS405 with an extended LEU2 marker This study pRS406red Modified version of pRS406 with an extended URA3 marker This study pFS181 Plasmid vector used as a template for amplifying hENT1. (4) pATP403red-hENT1 pATP403red vector with hENT1 downstream of pTDH3. ..
Expressing:Article Title: Robust and flexible platform for directed evolution of yeast genetic switches
Article Snippet: .. Plasmid name Relevant features Source pACYCDuetTM -1 Expression vector for E. coli Novagen pGK415 Expression vector containing pPGK1, TPGK1, the CEN/ARS replication origin, and the LEU2 marker (2) pRS403 Integration vector (without yeast replication origin) with the HIS3 marker ATCC pRS405 Integration vector (without yeast replication origin) with the LEU2 marker ATCC pRS406 Integration vector (without yeast replication origin) with the URA3 marker ATCC pRS416 Expression vector containing the CEN/ARS replication origin and URA3 marker ATCC pATP403 Genome integration vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the HIS3 marker (3) pATP406 Genome integration vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the URA3 marker (3) pATP426 Expression vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the URA3 marker (3) pATP403red Modified version of pATP403 with an extended HIS3 marker This study pRS403red Modified version of pRS403 with an extended HIS3 marker This study pRS405red Modified version of pRS405 with an extended LEU2 marker This study pRS406red Modified version of pRS406 with an extended URA3 marker This study pFS181 Plasmid vector used as a template for amplifying hENT1. (4) pATP403red-hENT1 pATP403red vector with hENT1 downstream of pTDH3. ..
Marker:Article Title: Robust and flexible platform for directed evolution of yeast genetic switches
Article Snippet: .. Plasmid name Relevant features Source pACYCDuetTM -1 Expression vector for E. coli Novagen pGK415 Expression vector containing pPGK1, TPGK1, the CEN/ARS replication origin, and the LEU2 marker (2) pRS403 Integration vector (without yeast replication origin) with the HIS3 marker ATCC pRS405 Integration vector (without yeast replication origin) with the LEU2 marker ATCC pRS406 Integration vector (without yeast replication origin) with the URA3 marker ATCC pRS416 Expression vector containing the CEN/ARS replication origin and URA3 marker ATCC pATP403 Genome integration vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the HIS3 marker (3) pATP406 Genome integration vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the URA3 marker (3) pATP426 Expression vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the URA3 marker (3) pATP403red Modified version of pATP403 with an extended HIS3 marker This study pRS403red Modified version of pRS403 with an extended HIS3 marker This study pRS405red Modified version of pRS405 with an extended LEU2 marker This study pRS406red Modified version of pRS406 with an extended URA3 marker This study pFS181 Plasmid vector used as a template for amplifying hENT1. (4) pATP403red-hENT1 pATP403red vector with hENT1 downstream of pTDH3. ..
Modification:Article Title: Robust and flexible platform for directed evolution of yeast genetic switches
Article Snippet: .. Plasmid name Relevant features Source pACYCDuetTM -1 Expression vector for E. coli Novagen pGK415 Expression vector containing pPGK1, TPGK1, the CEN/ARS replication origin, and the LEU2 marker (2) pRS403 Integration vector (without yeast replication origin) with the HIS3 marker ATCC pRS405 Integration vector (without yeast replication origin) with the LEU2 marker ATCC pRS406 Integration vector (without yeast replication origin) with the URA3 marker ATCC pRS416 Expression vector containing the CEN/ARS replication origin and URA3 marker ATCC pATP403 Genome integration vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the HIS3 marker (3) pATP406 Genome integration vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the URA3 marker (3) pATP426 Expression vector containing three sets of promoters/terminators (pADH1/TADH1, pTDH3/TTDH3, and pPGK1/TPGK1,) and the URA3 marker (3) pATP403red Modified version of pATP403 with an extended HIS3 marker This study pRS403red Modified version of pRS403 with an extended HIS3 marker This study pRS405red Modified version of pRS405 with an extended LEU2 marker This study pRS406red Modified version of pRS406 with an extended URA3 marker This study pFS181 Plasmid vector used as a template for amplifying hENT1. (4) pATP403red-hENT1 pATP403red vector with hENT1 downstream of pTDH3. ..
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